With the development of biotechnology, cell culture has become an essential process in order to study cell signal transduction, anabolism, growth and value-added. Cell culture refers to in vitro simulation in vivo environment (sterile, suitable temperature, pH and certain nutritional conditions, etc.), so that the survival, growth, reproduction and maintain the main structure and function of a method.

In the culture of animal cells usually need to useCO2This is supplemented by controlling the concentration of oxygen in the culture environment to better mimic the conditions in the body, because in the animal bodyO2The concentration range is1-14%This is related to the atmosphere.O2content is20-21% Different, so the cells cultured in hypoxic environment grow faster, live longer, and show lower stress response. Cell hypoxia culture is of great significance to understand the progress mechanism of major diseases such as tumor, organ fibrosis, brain injury and so on and to find preventive measures. Hypoxia incubator is an indispensable equipment to create hypoxic environment. Hypoxia incubator is a kind of hypoxic environment(O2Concentration

The importance of hypoxic environment for cell culture is well known, but for most researchers, it is extremely difficult to accurately control and monitor hypoxic conditions, because only precision instruments can accurately control the temperature, humidity and gas (CO2andO2). There are currently two ways to reduce the oxygen concentration in the environment.CO2Modular air-tight gas chambers are used in the incubator. By using such chambers placed in standard incubators, the oxygen concentration can be reduced in vitro. Another frequently used method is to carry out low-oxygen cultivation in the so-called "three-gas" incubator, usually by adding two gases, carbon dioxide and nitrogen, to reduce the oxygen content.

In order to accurately measure the oxygen concentration of the incubator, a high-precision oxygen probe can be built in the hypoxia incubator to monitor the gas concentration in the incubator and feed it back to the controller to automatically pump the gas to reach the preset gas concentration. The control feedback system can quickly restore and maintain the set oxygen level at any time according to the preset value. In order to maintain a constant culture condition, the oxygen sensor can be used to monitor the oxygen content in the incubator atmosphere in real time. However, there is an agent corresponding oxygen sensor. The fluorescent oxygen sensor LOX-02 is recommend used here. This is an oxygen sensor developed by SST in the UK that applies the principle of fluorescence quenching. It has a long service life and almost no cross sensitivity. Its own pressure and temperature compensation enable the sensor to realize linear operation over a wider temperature range. LOX-02 measures oxygen partial pressure ppO2 (mbar) and temperature (°C). The user can select a pressure sensor to measure atmospheric pressure at the same time, and then convert the oxygen partial pressure reading ppO2 into oxygen concentration.

The following isLOX-02Some parameters:

Supply voltage; 4.5 ~ 5.5VDC

Supply current:; peak current:；

      Output: 3.3V TTL level;

      Oxygen measurement range: ppO2: 0-300mbar; O2% :0 - 25%

Response time:

Accuracy:

Operating temperature:-30 ~ +60°C

Storage temperature: -30 ~ +60°C

Humidity:0 - 99% Rh (No condensation)

Pressure range: 500-1200mbar

If the CO2 concentration needs to be detected at the same time, it is recommend to use the NDIR carbon dioxide sensor ExplorIR-M, which is a small low-power NDIR CO2 sensor manufactured by GSS using the most advanced solid-state LED optical technology. Low-power LEDs are manufactured in-house, enabling GSS to fully control the CO2 sensor signal chain. The sensor is designed for applications that require reliable operation in a minimum of space under various conditions and environments, and can measure CO2 levels up to 100% concentration.

      The specific parameters are as follows:

For details, please consult

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